Figure 2 shows a collage consisting of two E. coli pathways overlaid with predicted reaction flux data. In this diagram, rather than drawing connecting lines, compounds that are shared between the two pathways are merged, showing glycolysis flowing seamlessly into fermentation.
Figure 3 depicts a collage containing a larger number of pathways at a lower zoom level, so metabolite, enzyme and gene names are automatically suppressed (the font size of the pathway labels has been increased so those labels remain visible). In addition to manually repositioning pathways, merging some common nodes, and changing the default colors, some metabolites of interest have been highlighted in purple.
Now that you've seen what you can do with a Pathway Collage, how can you create one for yourself? Pathway Collages can be created from either the BioCyc website (or other Pathway Tools-based website) or from desktop Pathway Tools. There are five basic steps.
- Specify the set of pathways to be included. The simplest and most reliable way to specify a set of pathways is to generate a SmartTable containing the desired pathways, and then export the SmartTable to a Pathway Collage. This works both for the desktop and web versions of Pathway Tools, and enables you to keep your list of pathways around in case you ever want to edit it or regenerate your collage. There are other ways to specify a set of pathways, such as by interactively clicking on them in the cellular overview diagram (desktop only), from an omics dataset (web only), or by creating a seed collage from a single pathway and then interactively adding more (web only). We may add additional options to specify pathways in the future. Consult the documentation for more details.
- Export to Pathway Collage Viewer. Pathway Tools will compute automatic layouts of the individual pathways within the collage, then position those diagrams next to one another horizontally, and send that initial layout of the collage to the Pathway Collage Viewer application in your web browser.
- Interactively refine and customize the collage. This can involve repositioning items, showing connections, adding, deleting or merging elements, editing labels, highlighting elements of interest, and/or customizing node and edge styles. By default, only the metabolites along the main backbone of a pathway are included in the diagrams, but side metabolites can be added interactively. Additional pathways involving a metabolite of interest can also be added interactively.
- Import omics data to be visualized on the collage (optional). Omics data can be added either before or after the collage is generated. The collage can display omics data associated with either genes, metabolites, or reactions. When multi-timepoint gene expression data is displayed, the display of enzyme names is suppressed.
- Save or export the collage. At any time, a pathway collage can be saved as a JSON-format graph file on your computer; that file can later be loaded back in to the collage viewer (not all browsers support this operation --- we recommend using Chrome or Firefox). A pathway collage can also be exported to a PNG-format image file for use in presentations or publications. The image will be generated with a resolution comparable to that of the display at the time the image is created (up to some maximum), therefore, the highest-quality images are obtained if the collage is displayed at a high zoom level when exporting.